Samuel Natelson begins the interview with a discussion of his family background and childhood in Brooklyn, New York. He attended City College of New York and received his BS in chemistry in 1928. As a graduate student, Natelson attended New York University, receiving a ScM in 1930 and his PhD in 1931. After receiving his PhD, he began his career teaching at Girls Commercial High School. While maintaining his teaching position, Natelson joined the Jewish Hospital of Brooklyn in 1933. Working as a clinical chemist for Jewish Hospital, Natelson first conceived of the idea of a society by and for clinical chemists. Natelson worked to organize the nine charter members of the American Association of Clinical Chemists, which formally began in 1948. A pioneer in the field of clinical chemistry, Samuel Natelson became a role model for the clinical chemist. Natelson developed the usage of microtechniques in clinical chemistry. During this period, he served as a consultant to the National Aeronautics and Space Administration in the 1960s, helping analyze the effect of weightless atmospheres on astronauts' blood. Natelson spent his later career as chair of the biochemistry department at Michael Reese Hospital and as a lecturer at the Illinois Institute of Technology. He then became an adjunct professor at the University of Tennessee's College of Veterinary Medicine. Natelson concludes his interview with thoughts on the future of clinical chemistry and reflections on his career and family.